LCM: LASER CAPTURE MICRODISSECTION;
Principle introduction: under the action of the energy difference formed by the laser pulse, the edge of the tissue is cut off and separated, the tissue is instantly melted and adhered to the target under the action of the infrared laser, and the target is adsorbed on a specific collection cap.
Application introduction: After magnification of high-resolution microscope, specific subcellular structures in the image can be directionally separated and captured under bright-field and fluorescent-field conditions. It can be applied to the apparent genome of biological samples such as microorganisms, animals and plants, viruses, Single component study of transcriptomics and proteomics.
Features: This method is based on physical separation, with less damage to the tissue, high target purity, and high industry recognition. Due to the current level of operator and automation, the throughput is relatively small compared to other platforms.
Main instrument platform: LEICA (http://ppc.leica-microsystems.com.cn/) ABI (https://www.thermofisher.com) MMI (http://www.molecular-machines.com/home)
Other similar single-component separation equipment: C1 platform (cn.fluidigm.com), BD streaming platform (http://www.bdbiosciences.com), 10X Genomics platform (https://www.10xgenomics.com/)
Methodological common path: Take animal tissue as an example, tissue acquisition --- clear morphological identification standards --- slice preparation --- target capture-verify application experiments, low-temperature operation and dry environment have an important impact on the success of the experiment .
Project experience advantages: (clinical samples are taken as an example) The company has cooperated with many well-known key universities in the country such as Dongfang Hepatobiliary Hospital of Naval Military Medical University, Zhejiang University, Fourth Military Medical University, and Shanghai Jiaotong University to conduct relevant research, and the research target organs cover bile duct cancer , Lung cancer, pancreatic cancer, ovarian cancer, throat cancer, heart valve disease, etc. Applications include spatial genomics association studies of paraffin tissue, spatial transcriptomics of frozen or fresh tissue, and all have been validated. Our project members include engineers with a background in pathology and next-generation sequencing technology. We welcome researchers or enthusiasts with a background in proteomics to develop proteomics applications with us. The following are our methodological illustrations and case illustrations. For good separation results and other omics applications, please contact us and cooperate, and welcome your inquiry! Email: 657951487@qq.com
freedombiotech@gmail.com Tel: 086-15921707948 wechat: ARES657951487 WEB: www.freedombio.com

                 (Eg.feiai lung cancer)